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Death caused by the absence of Cys was rescued by the addition of free radical scavengers ferrostatin-1 (Fer-1) and N-acetyl-cysteine (NAC) but not by inhibition of caspase-dependent apoptosis by z-VAD-fmk or necroptosis by Nec1s (Fig 3A and B), indicating that Cys starvation induced ferroptosis in ES cells

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Since the pad2-1 mutant is severely deficient in GSH content, we supplemented these mutant seedlings with exogenous GSH or DTT